| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Bacillus thuringiensis | TCGGAGGACAAGAACCAGG | TTGCGGTGCAAAACGTCC | 58.64 | 59.59 | 236 | 118 |
100.00%
|
16.67%
|
| Primer 2 | Bacillus thuringiensis | TTCGGAGGACAAGAACCAGG | TTGCGGTGCAAAACGTCC | 59.31 | 59.59 | 237 | 118 |
100.00%
|
16.67%
|
| Primer 3 | Bacillus thuringiensis | ACTGGCCAAAGCACTGAGT | TACGACTGCCCTACCTGCA | 59.47 | 60.30 | 156 | 102 |
100.00%
|
27.78%
|
| Primer 4 | Bacillus thuringiensis | TTGCACAGCAAGTTCGCC | TGAGCAGCTTGGCGAACA | 59.28 | 59.89 | 268 | 101 |
91.67%
|
61.11%
|
| Primer 5 | Bacillus thuringiensis | TCCATGAACTTGGGCATGC | TCCGCATGAAGCCAACCA | 58.42 | 59.57 | 214 | 99 |
100.00%
|
22.22%
|
| Primer 6 | Bacillus thuringiensis | AGCGAATGGGCATGCAAC | TGTTTGCGCTGTAACTTCCTG | 59.11 | 59.40 | 217 | 94 |
100.00%
|
16.67%
|
| Primer 7 | Bacillus thuringiensis | AGCGAATGGGCATGCAAC | TGCGCTGTAACTTCCTGAAAC | 59.11 | 59.13 | 213 | 94 |
100.00%
|
16.67%
|
| Primer 8 | Bacillus thuringiensis | CCGTGGACGCATTGAAGTG | TGAGCAGCTTGGCGAACA | 59.50 | 59.89 | 252 | 94 |
90.91%
|
55.56%
|
| Primer 9 | Bacillus thuringiensis | TCCATGAACTTGGGCATGCT | TCCGCATGAAGCCAACCA | 59.96 | 59.57 | 214 | 92 |
100.00%
|
22.22%
|
| Primer 10 | Bacillus thuringiensis | CCATGAACTTGGGCATGCT | TCCGCATGAAGCCAACCA | 58.41 | 59.57 | 213 | 92 |
100.00%
|
22.22%
|